От изданията на ЦМБ / CML-Journal Publications
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С тази рубрика улесняваме достъпа и насочваме вниманието към избрани статии, вече публикувани в наши издания. Подборът е читателски или редакционен, с основни критерии – значимостта на проблема и дефицитът на достъпна актуална литература по въпроса у нас. Publications from scientific medical periodicals, edited in Central Medical Library (CML)- MU Sofia
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Търсене От изданията на ЦМБ / CML-Journal Publications от Автор "Ananiev, J."
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- ДокументHPV-associated Buschke-Löwenstein tumours in a patient with disseminated anogenital form of condylomata acuminata – a dermatosurgical problem(Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia, 2012-06) Tchernev, G.; Ananiev, J.; Gulubova, M.; Bakardzhiev, I.; Pehlivanov, G.; Gonevski, M.; Zisova, L.; Cardoso, J. C.Buschke-Löwenstein tumours are a relatively rare clinicopathological entity. However, due to their malignant potential and frequent association with some types of Human Papillomaviruses (HPV), their diagnosis should be promptly confi rmed and appropriate management should be planned (according to the condition). In this article we present a middle-aged patient with long-standing tumoural lesions located in the anogenital area, which were confi rmed clinically and histopathologically as well-differentiated squamous cell carcinomas of Buschke-Löwenstein type, in combination with disseminated anogenital condylomata acuminata. Histologically, large tumour nests of well-differentiated squamous cell carcinoma were observed, as well as a marked mononuclear cell infi ltrate and conspicuous koilocytosis. Immunohistochemistry for Ki-67 revealed a relatively high proliferative index, and was strongly positive for HPV with a pan-HPV antibody (AK), confi rming the presence of the virus in the tissue. Surgical removal of the various tumoural masses was performed by electrodesiccation, combined with shave-curettage under general anesthesia. Due to the excessive bleeding there was no possibility for complete removal of the perianal tumour formations and the therapeutic strategy was revised. Systemic therapy with interferon was planned with the purpose of reducing the tumour mass before further surgery.
- ДокументOncogenic balanitis(Централна медицинска библиотека - МУ София / Central Medical Library - MU Sofia, 2013-09) Tchernev, G.; Ananiev, J.; Cardoso, J. C.; Philipov, S.; Wollina, U.Summary. The so-called „oncogenic balanitis“ is a not so rare nosological entity, whose pathogenesis is related in most cases to infection with oncogenic strains of the human papilloma viruses (HPVs). In the early stages of the disease the clinical presentation of the infection with some oncogenic or high risk human HPVs is often non-specific, contributing to its persistance for a long time, often misdiagnosed as a candidous or bacterial balanitis. Clinically the lesions may also be confused with lichen planus or lichen sclerosus, as well as penile eczema or psoriasis. Local and/or systemic therapy with corticosteroids can lead to unmasking of the symptoms and rapid progression of the disease, with potential risk of malignant transformation. Additionally, some patients decline a biopsy, further hampering the confirmation of the diagnosis. In all these problematic cases a diagnostic PCR examination of material taken from lesional skin could be of help for the diagnosis of Bowen’s disease, erythroplasia Queyrat, consequently preventing its progression to invasive squamous cell carcinoma. We present a rare case of erosive and partially verrucous balanitis, in which HPV 56 strains were identifed by PCR in repeated samples from lesional skin. Additionaly, HPV 16 was also identified in the second sample. The clinical findings were entirely consistent with erythroplasia of Queyrat but histopathological examination was not possible. A local therapy with imiquimod was started. After complete clinical recovery a vaccination with Gardasil was additionaly planned with the hope that the patient would be protected from possible recurrence due to the generation of cross-immunity against HPV 56. Due to the risk of malignant transformation into squamous cell carcinoma of the penis, HPV-associated “oncogenic balanitis” is a disease portending a potentially unfavorable prognosis. In patients with longstanding genital lesions unresponsive to topical treatments usually used for balanitis, the possibility of HPV-induced “oncogenic balanitis” should be a strong consideration. PCR testing from lesional skin is probably one of the most sensitive, accurate, and reliable methods that help the clinician identify oncogenic and nononcogenic HPV-strains in problematic patients who refuse invasive and semi-invasive diagnostic options such as biopsy and cytological examination. Subsequent therapy should be complemented by regular clinical follow-up due to the possibility of relapse.
- ДокументA rare case of a patient with a metatypical basal cell carcinoma of the forehead treated successfully with elliptical excision(Централна медицинска библиотека - МУ София / Central Medical Library - MU Sofia, 2013-09) Tchernev, G.; Ananiev, J.; Cardoso, J. C.Summary: We present a rare case of a 72-year-old patient with an ulcerative lesion localized to the left forehead, appearing 3 months prior to clinical presentation. Biopsy revealed a metatypical basal cell carcinoma. Treatment consisted of wide local excision. There was no sign of recurrence or metastasis after a 12-month follow-up period. Basal cell carcinoma (BCC) and squamous cell carcinoma (SCC) make up 95% of the most common type of cancer in the world, the non-melanoma skin cancer. BCC is known for its potential to be locally invasive, while SCC for its potential to metastasize in lymph nodes. Metatypical basal cell carcinoma (MTBC) is a rare tumor that combines clinical and histopathological features of both BCC and SCC. The gold standard for diagnosis lies in the histopathologic examination. Clinical examination alone does not suffi ce.
- Документр53 – “the guardian of genome”(Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia, 2011-10) Ananiev, J.; Tchernev, G.; Patterson, J. W.; Gulubova, M.; Ganchev, G.p53 is a transcription-suppressing factor, which participates in control of cell cycle and apoptosis and in the regulation of cell genomic integrity. p53 is a tumor suppressor protein that in humans is encoded by the TP53 gene. p53 is crucial in multicellular organisms, where it regulates the cell cycle and, thus, functions as a tumor suppressor that is involved in preventing cancer. The name p53 is in reference to its apparent molecular mass: It runs as a 53-kilodalton (kDa) protein on SDS-PAGE. But, based on calculations from its amino acid residues, p53's mass is actually only 43.7 kDa. This difference is due to the high number of proline residues in the protein, which slow its migration on SDS-PAGE, thus making it appear heavier than it actually is. This effect is observed with p53 from a variety of species, including humans, rodents, frogs, and fi sh. Mutations of p53 are observed in 50% of all human carcinomas and in 90% of melanomas being the most frequently genome disturbance in carcinomas. Also, allele loss and mutation of the p53 gene are detected in more than 60% of gastric cancers regardless of histological type. Different mechanisms of activation, physiological functions and the role of p53 in cancerogenesis and in different neoplasms are viewed. Immunohistochemical recognition of p53 in normal cells and neoplastic altered cells gives information about genesis, prognosis and sensitivity to therapy of different types of cancers. Nevertheless, prospective studies need to address this issue definitely and this marker need further investigation and confirmation.