Role of leptin in oxidative stress and survival of human granulosa luteinized cells in vitro
| dc.contributor.author | Dineva, J. | |
| dc.contributor.author | Vangelov, I. | |
| dc.contributor.author | Rangelov, I. | |
| dc.contributor.author | Stamenov, G. | |
| dc.contributor.author | Fartsova, T. | |
| dc.date.accessioned | 2015-08-07T13:59:10Z | |
| dc.date.available | 2015-08-07T13:59:10Z | |
| dc.date.issued | 2013-09 | |
| dc.description | Address for correspondence: Assoc. Prof. Julieta Dineva, PhD; Institute of Biology and Immunology; of Reproduction “Acad. K. Bratanov”, BAS; 73, Tsarigradsko shosse Blvd.; 1113 Sofia, Bulgaria; e-mail: julietadineva@yahoo.com | en_US |
| dc.description.abstract | Summary. The aim of this study was to investigate the effect of leptin on oxidative status and survival of human granulosa luteinized cells (GLCs) in vitro. The human GLCs were isolated from follicular fl uids of women undergoing IVF procedure and cultured in DMEM /HAM F12 with 10% FCS for 24 h. To study the oxidative status and survival of GLCs, after a 24-hour culture period, the cells were supplemented with 0, 1, 10, 100 ng/ml leptin in DMEM/ HAM F12 plus 1% FCS and cultured for another 24 h. The level of malondialdehyde (MDA), formed during the decomposition of lipid peroxidation products in cells, was measured by TBARS Assay Kit. Using the specifi c substrate (AcDEVD-PNA), the caspase-3 activity was measured as an indicative marker for apoptosis. Treatment with a physiological dose of leptin (10 ng/ml) decreased MDA level, while the higher dose of leptin (100 ng/ml) resulted in increased MDA level, compared to control cells (p < 0.05). Moreover, the treatment of GLCs with 100 ng/ml of leptin led to a significant increase in caspase-3 activity compared to control cells or cells treated with 10 ng/ml leptin (p < 0.05). The higher concentration of MDA was related to higher caspase-3 activity (R = 0.5789, p = 0.049). In conclusion, leptin plays an important role in oxidative metabolism and survival of human granulosa luteinized cells. | en_US |
| dc.identifier.citation | J. Dineva, I. Vangelov, I. Rangelov, G. Stamenov and T. Fartsova. Role of leptin in oxidative stress and survival of human granulosa luteinized cells in vitro - Acta medica bulgarica , 40, 2013, No 2, 33-38. | en_US |
| dc.identifier.issn | 0324-1750 | |
| dc.identifier.uri | http://hdl.handle.net/10861/724 | |
| dc.language.iso | en | en_US |
| dc.publisher | Централна медицинска библиотека - МУ София / Central Medical Library - MU Sofia | en_US |
| dc.subject | apoptosis, human granulosa luteinized cells, leptin, oxidative stress | en_US |
| dc.title | Role of leptin in oxidative stress and survival of human granulosa luteinized cells in vitro | en_US |
| dc.type | Article | en_US |