Methods of protein delivery into mammalian cells for gene therapy and genetic studies
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Дата
2008-06
Автори
Todorova, R.
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Издател
Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia
Резюме
Methods, lying on different principles for introducing proteins into mammalian cells were compared in the present study. Some of them rely on non-covalent complex formation (Chariot), osmotic lysisis of pinocytic vesicles (Influx pinocytic cell-loading reagent), electric power (electroporation), lipid-based delivery system (Bioporter), microinjection, small protein transduction domains (PTDs) from viral proteins, bacterial secretion system type III (T3SS). Thus, the delivery by many of the compared methods can give a sufficient number of uniformly loaded cells for different studies including transcription and translation, cell cycle regulation, control of apoptosis, oncogenesis and gene therapy.
Описание
Address for correspondence:
R. Todorova
Institute of Biophysics
Bulgarian Academy of Sciences
1113 Sofia
Bulgaria
02 979-31-25
e-mail: todorova@obzor.bio21.bas.bg
Ключови думи
protein delivery, mammalian cells, gene therapy, electroporation, bio-porter, microinjection, nanoparticles, cell-penetrating peptides, pinocytic vesicles
Цитирания
Todorova, R. Methods of protein delivery into mammalian cells for gene therapy and genetic studies - Acta Medica Bulgarica, 35, 2008, № 1, 3-11.