Methods of protein delivery into mammalian cells for gene therapy and genetic studies
| dc.contributor.author | Todorova, R. | |
| dc.date.accessioned | 2012-05-04T12:44:28Z | |
| dc.date.available | 2012-05-04T12:44:28Z | |
| dc.date.issued | 2008-06 | |
| dc.description | Address for correspondence: R. Todorova Institute of Biophysics Bulgarian Academy of Sciences 1113 Sofia Bulgaria 02 979-31-25 e-mail: todorova@obzor.bio21.bas.bg | en_US |
| dc.description.abstract | Methods, lying on different principles for introducing proteins into mammalian cells were compared in the present study. Some of them rely on non-covalent complex formation (Chariot), osmotic lysisis of pinocytic vesicles (Influx pinocytic cell-loading reagent), electric power (electroporation), lipid-based delivery system (Bioporter), microinjection, small protein transduction domains (PTDs) from viral proteins, bacterial secretion system type III (T3SS). Thus, the delivery by many of the compared methods can give a sufficient number of uniformly loaded cells for different studies including transcription and translation, cell cycle regulation, control of apoptosis, oncogenesis and gene therapy. | en_US |
| dc.identifier.citation | Todorova, R. Methods of protein delivery into mammalian cells for gene therapy and genetic studies - Acta Medica Bulgarica, 35, 2008, № 1, 3-11. | en_US |
| dc.identifier.issn | 0324-1750 | |
| dc.identifier.uri | http://hdl.handle.net/10861/143 | |
| dc.language.iso | en | en_US |
| dc.publisher | Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia | en_US |
| dc.subject | protein delivery, mammalian cells, gene therapy, electroporation, bio-porter, microinjection, nanoparticles, cell-penetrating peptides, pinocytic vesicles | en_US |
| dc.title | Methods of protein delivery into mammalian cells for gene therapy and genetic studies | en_US |
| dc.type | Article | en_US |