BCR/ABL molecular testing by fl uorescence in situ hybridization in untreated adult acute leukemia
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Дата
2012-06
Автори
Velizarova, M.
Dimova, I.
Hadzhiev, E.
Tsatchev, K.
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Издател
Централна медицинска библиотека, МУ София / Central Medical Library - MU Sofia
Резюме
The BCR/ABL detection infl uences diagnosis, prognosis and treatment
decisions in patients with chronic myeloid leukemia (CML) and acute lymphoblastic
leukemia (ALL). We studied the feasibility and the sensitivity of LSI BCR/ABL
dual-colour, dual-fusion probe as a usual assay for detection BCR-ABL gene fusion
in acute leukemia. A total of 3229 successfully hybridized interphase nuclei from 34
newly diagnosed patients with acute leukemias (12 AML and 22 ALL cases) were analyzed.
We used interphase fl uorescence in situ hybridization (FISH) method and LSI
BCR/ABL dual-colour, dual-fusion probe (Vysis) for detection of BCR-ABL gene that
span the common breakpoints of t(9;22)(q34;q11.2) and that detect double BCR-ABL
fusion in bone marrow cells with this translocation, one on the abnormal chromosome
9 and one on the Philadelphia chromosome (Ph chromosome). Fusion BCR-ABL
gene was observed in 9 (28.13%) of the cases with successful hybridization and in
6 (18.75%) of them the results had clinical reliability. The aberrant cell lines were of
different sizes − 53%-96% of cells were positive for BCR-ABL gene. Fusion signals
for BCR-ABL were reported in 2 (16.67%) of AML and in 4 (18.18%) of ALL cases.
In conclusion, we proved that LSI BCR-ABL dual-colour, dual-fusion probe (Visys) is
characterized with high specifi city and sensibility.
Описание
Address for correspondence:
Dr. Milena Velizarova;
Department of Clinical Laboratory and Clinical Immunology;
University Hospital “Alexandrovska”;
1 G. Sofi yski str.;
1431 Sofi a, Bulgaria;
tel.: +359 2 92 30 916 ;
e-mail: mvelizarova@abv.bg
Ключови думи
Interphase FISH, BCR-ABL gene, adult acute leukemia
Цитирания
M. Velizarova, I. Dimova, E. Hadzhiev and K. Tsatchev. BCR/ABL molecular testing by fl uorescence in situ hybridization in untreated adult acute leukemia - Acta medica bulgarica , 39, 2012, No 2, 82-88.